Coding

Part:BBa_K3786000:Design

Designed by: Camila Ballenghien, Nathalia Fernandes   Group: iGEM21_Paris_Bettencourt   (2021-10-21)


Filamenting temperature-sensitive mutant Z


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 112
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 112
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 112
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 112
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 112
    Illegal AgeI site found at 322
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the part is compatible with enzyme type IIS assembly.


Source

It comes from the genome of E. Coli

References